Книга: Hymonti Dey and Sudip Debnath «Detection of Tuberculosis By Polymerase Chain Reaction (PCR)»
|
Производитель: "LAP Lambert Academic Publishing" The polymerase chain reaction (PCR) has recently been investigated for detecting Mycobacterium tuberculosis in clinical specimens and appears to have significant potential as a rapid, sensitive, and specific assay for diagnosing infections caused by this organism. One of the remaining issues regarding the use of this technique is the extraction of DNA from clinical material prior to performing the PCR. It requires the use of DNA extraction method that can efficiently lyse mycobacterial cells and recover small amounts of DNA. The method of heating the sample in a boiling water bath to break down the bacterial cell wall and to release the DNA was compared with that of enzymatic lysis of bacteria and then phenol-chloroform extraction of DNA (Modified chemical method). Modified chemical method was better than just boiling the sample to remove DNA for amplification with PCR. In this study 140 sputum samples were collected from suspected prisoners at Dhaka Central Jail. All of those samples... ISBN:9783659161445 Издательство: "LAP Lambert Academic Publishing" (2012)
ISBN: 9783659161445 |
См. также в других словарях:
Tuberculosis diagnosis — Tuberculosis is diagnosed by finding Mycobacterium tuberculosis bacteria in a clinical specimen taken from the patient. While other investigations may strongly suggest tuberculosis as the diagnosis, they cannot confirm it.=Diagnosis=A complete… … Wikipedia
Applications of PCR — This page assumes familiarity with the terms and components used in the Polymerase Chain Reaction (PCR) process. The Polymerase Chain Reaction (PCR) has found widespread application in many areas of genetic analysis. This is a list of some of… … Wikipedia
Mycobacterium ulcerans — Scientific classification Kingdom: Bacteria Phylum: Actinobacteria Order: Actinomycetales … Wikipedia
Nobel Prize controversies — Nobel Prize Awarded for Outstanding contributions in physics, chemistry, literature, peace, and physiology or medicine. The Sveriges Riksbank Prize in Economic Sciences in Memory of Alfred Nobel, identified with the Nobel Prize, is awarded for… … Wikipedia
HIV test — Randall L. Tobias, former U.S. Global AIDS Coordinator, being publicly tested for HIV in Ethiopia in an effort to reduce the stigma of being tested.[1] HIV tests are used to detect the presence of the human immunodeficiency virus (HIV), the virus … Wikipedia
Infectious disease — Classification and external resources A false colored electron micrograph shows a malaria sporozoite migrating through the midgut epithelia. ICD 10 … Wikipedia
