Книга: Wondu Garoma Berra «Analysis of lycopene in tomato (Lycopersicon esculentum, Mill.) fruits»

Производитель: "LAP Lambert Academic Publishing"

Lycopene is highly unsaturated, open-chain carotenoid found abundantly in tomatoes. Research interests into properties&health benefits of lycopene have been growing, bringing attention to content of lycopene in food. The purpose of this work was to develop efficient&accurate protocol that can measure lycopene using HPLC, and establish relationship with Vis/NIR reflectance spectroscopy measurement. Accordingly, mobile phase composition ACN/MeOH (50:50, v/v)+ TEA 9 M; extraction solvents hexane/acetone/ethanol (50:25:25, v/v/v); re-dissolving residue in THF, followed ACN/MeOH (15:30:55, v/v/v); flow rate 0. 6 mL/min and ?detection 472 nm were showed most suitable for lycopene determination in tomato. This method has achieved best characteristic spectra profile with precision (RSD>15), accuracy&recovery (? 81. 7%) and sensitive detection limit (0. 0156 ?g/mL) within separation of 21 minutes. Finally, the established method was applied to selected tomato cultivars and a wide... ISBN:9783847327486

Издательство: "LAP Lambert Academic Publishing" (2011)

ISBN: 9783847327486