Книга: Jehan Alam and Najmul Hasan «A New dot-ELISA Kit»

Производитель: "LAP Lambert Academic Publishing"

Poultry farming is the most dynamic&2nd major industry in Pakistan. It has challenges of outbreaks of viral diseases resulting in high mortality, morbidity and huge economic loss in Pakistan. Conventional diagnostic tests are expensive, laborious&require technical expertise. Hence, a highly sensitive&specific dot-ELISA kit is developed, in two steps; first whole antigen is prepared from AIV, NDV and IBV and is applied onto nitrocellulose membrane; second rabbit anti-chicken antibodies is raised, purified and conjugated with enzyme HRP. Dot ELISA results are much comparative with ELISA&have advantages of high specificity, sensitivity&facile. This kit has the advantage of loading multiple antigens from local isolates&can be performed even at poultry forms; with no technical expertise or advance laboratory facilities for interpretations. Even the staff can test their bird s immune response after vaccination or test for the presence of infection. It can be prepared&used... ISBN:9783659403088

Издательство: "LAP Lambert Academic Publishing" (2013)

ISBN: 9783659403088