Книга: Vibhor Gupta and Rameshwar N.K. Bamezai «Functional Relevance of Human Pyruvate Kinase-M2 Mutations»
Производитель: "LAP Lambert Academic Publishing" Out of 4 isoforms of Pyruvate kinase; a glycolytic enzyme, M2 is a prototype isoform expressed in embryonic and adult dividing cells. For the first time, we reported two missense mutations in Pyruvate kinase M2 isozyme (H391Y and K422R) from the cells of Bloom syndrome patients, prone to develop cancer. Results showed that despite the presence of mutations in the inter-subunit contact domain, the K422R and H391Y mutant proteins maintained their homotetrameric structure, similar to the wild-type protein, but showed a loss of activity by 75 and 20%, respectively. Interestingly, H391Y showed a 6-fold increase in affinity for its substrate phosphoenolpyruvate and behaved like a non-allosteric protein with compromised cooperative binding. The co-expression of homotetrameric wild type and mutant PKM2 in the cellular milieu resulted in the interaction between the two at the monomer level, which was substantiated further by in vitro experiments. Interestingly, in a unique observation,... ISBN:9783848408962 Издательство: "LAP Lambert Academic Publishing" (2012)
ISBN: 9783848408962 |
См. также в других словарях:
Adenylate kinase — 3D ribbon/surface model of adenylate kinase in complex with bis(adenosine)teraphosphate (ADP ADP) Identifiers Symbol ADK … Wikipedia
Titin — The three dimensional structure of a type I module from titin. PDB rendering based on 1bpv … Wikipedia
Coenzyme Q10 — IUPAC name 2 [(2E,6E,10E,14E,18E,22E,26E,30E,34E) 3,7,11,15,19,23,27,31,35,39 … Wikipedia
Carnitine palmitoyltransferase I — carnitine palmitoyltransferase 1A (liver) Identifiers Symbol CPT1A Alt. symbols CPT1 Entrez 1374 … Wikipedia
Enzyme — Biocatalyst redirects here. For the use of natural catalysts in organic chemistry, see Biocatalysis. Human glyoxalase I. Two zinc ions that are needed for the enzyme to catalyze its reaction are shown as purp … Wikipedia